This is course #1 of a 5-part fundamentals of RT-qPCR series. These courses will give you an understanding of basic RT-qPCR techniques and troubleshooting for each step of the gene expression workflow.

 

Courses are led by Bio-Rad's Field Application Specialists, who have diligently compiled all the questions they have received in the field and have designed this program to be both comprehensive and efficient.

 

You will receive a downloadable certificate upon successful completion of each course.

What you will learn

in Course #1: Introduction to RT-qPCR and Gene Expression

  • DNA structure and the Polymerase Chain Reaction

  • Reagents and conditions considerations for DNA amplification during qPCR

  • Theory of fluorescence detection during qPCR

  • Understanding the shape and parts of an amplification curve

Course Overview

This is a preview, please enroll to access these lessons

    1. Welcome to the course

    2. How to use this course

    3. Meet the instructor

    1. Qualitative vs Quantitative Analysis Polymerase Chain Reaction

    2. Quiz #1

    1. DNA Structure and Reagent Considerations for DNA Amplification

    2. Quiz #2

    1. Temperature Considerations for DNA Amplification

    2. Quiz #3

    1. PCR Detection Chemistries SYBR Green

    2. Quiz #4

    1. PCR Detection Chemistries Probes

    2. Quiz #5

About this course

  • Free
  • 17 lessons
  • 0.5 hours of video content

Meet your instructor

Field Application Specialist David Eaves

David Eaves is an expert in molecular biology techniques, supported by over 15 years of hands-on experience working in both research and clinical environments. He has translated this experience to support researchers as a field application scientist and technical sales specialist at Bio-Rad Laboratories. He also spent 8 years at Cincinnati Children's Hospital Medical Center, serving as a senior molecular pathology technician, supporting clinical sequencing applications.

Reviews for this course

5 star rating

Good introduction to RT-qPCR and Gene Expression

Alex Prima

Short and easy to understand. Great starting point to trigger back my memory for what I've learned in the classroom and practical courses 7+ years ago. Thank...

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Short and easy to understand. Great starting point to trigger back my memory for what I've learned in the classroom and practical courses 7+ years ago. Thank you!

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5 star rating

Interesting and precise

David Larrea

A well structured and direct presentation of important information about the basic concepts of RT-qPCR. Very useful and good explanations.

A well structured and direct presentation of important information about the basic concepts of RT-qPCR. Very useful and good explanations.

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5 star rating

So interesting

Syahruni Hidayatullah

Course material is very interesting, simple, easy to understand and very clear

Course material is very interesting, simple, easy to understand and very clear

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5 star rating

Rt-pcr

HONSO TCHONANG Eric Christel

Five stars

Five stars

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FAQ

  • Who should take this course?

    This course is designed for people who want to have a fundamental understanding of RT-qPCR and gene expression.

  • How long will this course take?

    This class should take approximately 1 to 1.5 hours to complete.

  • Can I get a certificate for this course?

    Once all sections of this course have been completed a certificate with your name and course name will be available for download.

  • Will I be able to ask questions and get answers?

    By enrolling in this class you will be automatically added to Bio-Rad's RT-qPCR and Gene Expression community. This is a highly collaborative place to ask questions and share ideas with the instructors and your peers. You can post pictures, comments and reply to others.

  • What are the other classes in this series?

    There are five classes in the "Fundamentals of RT-qPCR and Gene Expression" series. Course 1: Introduction to RT-qPCR and gene expression, Course 2: RNA sample preparation considerations, Course 3: Experimental design for gene expression, Course 4: Assay optimization, Course 5: Gene expression analysis

  • Do I have to take the classes in order?

    It is recommended that you take the 5 fundamentals of RT-qPCR and gene expression classes in order. There are concepts introduced in earlier classes that are referred to in the later classes.