Introduction to RT-qPCR and Gene Expression
Course #1 of the Fundamentals of RT-qPCR Series
This is course #1 of a 5-part fundamentals of RT-qPCR series. These courses will give you an understanding of basic RT-qPCR techniques and troubleshooting for each step of the gene expression workflow.
Courses are led by Bio-Rad's Field Application Specialists, who have diligently compiled all the questions they have received in the field and have designed this program to be both comprehensive and efficient.
You will receive a downloadable certificate upon successful completion of each course.
Course Overview
This is a preview, please enroll to access these lessons
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Welcome to the course
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How to use this course
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Meet the instructor
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Qualitative vs Quantitative Analysis Polymerase Chain Reaction
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Quiz #1
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DNA Structure and Reagent Considerations for DNA Amplification
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Quiz #2
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Temperature Considerations for DNA Amplification
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Quiz #3
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PCR Detection Chemistries SYBR Green
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Quiz #4
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PCR Detection Chemistries Probes
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Quiz #5
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About this course
- Free
- 17 lessons
- 0.5 hours of video content
Meet your instructor
Field Application Specialist David Eaves
David Eaves is an expert in molecular biology techniques, supported by over 15 years of hands-on experience working in both research and clinical environments. He has translated this experience to support researchers as a field application scientist and technical sales specialist at Bio-Rad Laboratories. He also spent 8 years at Cincinnati Children's Hospital Medical Center, serving as a senior molecular pathology technician, supporting clinical sequencing applications.
Reviews for this course
Good introduction to RT-qPCR and Gene Expression
Alex Prima
Short and easy to understand. Great starting point to trigger back my memory for what I've learned in the classroom and practical courses 7+ years ago. Thank...
Read MoreShort and easy to understand. Great starting point to trigger back my memory for what I've learned in the classroom and practical courses 7+ years ago. Thank you!
Read LessInteresting and precise
David Larrea
A well structured and direct presentation of important information about the basic concepts of RT-qPCR. Very useful and good explanations.
A well structured and direct presentation of important information about the basic concepts of RT-qPCR. Very useful and good explanations.
Read LessSo interesting
Syahruni Hidayatullah
Course material is very interesting, simple, easy to understand and very clear
Course material is very interesting, simple, easy to understand and very clear
Read LessRt-pcr
HONSO TCHONANG Eric Christel
Five stars
Five stars
Read LessJust Fantastic!
Muhammad Umar
It covered all core concepts of PCR, qRTPCR. Love and gonna move to the next courses.
It covered all core concepts of PCR, qRTPCR. Love and gonna move to the next courses.
Read LessGood course for Basic understanding
Anagha Raviprakash
It posed a few challenging questions and is really good for undergrads like myself
It posed a few challenging questions and is really good for undergrads like myself
Read LessQuality content
Apoorva Jnana
Clear explanations, engaging quiz sessions.
Clear explanations, engaging quiz sessions.
Read LessPerfect introduction to RT-qPCR
Laiqa Anjum
This course is perfect to brush up your RT-QPCR skills, presentations and lectures have been summarised precisely and to the point.
This course is perfect to brush up your RT-QPCR skills, presentations and lectures have been summarised precisely and to the point.
Read LessFAQ
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Who should take this course?
This course is designed for people who want to have a fundamental understanding of RT-qPCR and gene expression.
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How long will this course take?
This class should take approximately 1 to 1.5 hours to complete.
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Can I get a certificate for this course?
Once all sections of this course have been completed a certificate with your name and course name will be available for download.
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Will I be able to ask questions and get answers?
By enrolling in this class you will be automatically added to Bio-Rad's RT-qPCR and Gene Expression community. This is a highly collaborative place to ask questions and share ideas with the instructors and your peers. You can post pictures, comments and reply to others.
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What are the other classes in this series?
There are five classes in the "Fundamentals of RT-qPCR and Gene Expression" series. Course 1: Introduction to RT-qPCR and gene expression, Course 2: RNA sample preparation considerations, Course 3: Experimental design for gene expression, Course 4: Assay optimization, Course 5: Gene expression analysis
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Do I have to take the classes in order?
It is recommended that you take the 5 fundamentals of RT-qPCR and gene expression classes in order. There are concepts introduced in earlier classes that are referred to in the later classes.
All Courses
In the Fundamentals of RT-qPCR and Gene Expression series