Introduction to RT-qPCR and Gene Expression
Course #1 of the Fundamentals of RT-qPCR Series
This is course #1 of a 5-part fundamentals of RT-qPCR series. These courses will give you an understanding of basic RT-qPCR techniques and troubleshooting for each step of the gene expression workflow.
Courses are led by Bio-Rad's Field Application Specialists, who have diligently compiled all the questions they have received in the field and have designed this program to be both comprehensive and efficient.
You will receive a downloadable certificate upon successful completion of each course.
Course Overview
This is a preview, please enroll to access these lessons
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Welcome to the course
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How to use this course
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Meet the instructor
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Qualitative vs Quantitative Analysis Polymerase Chain Reaction
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Quiz #1
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DNA Structure and Reagent Considerations for DNA Amplification
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Quiz #2
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Temperature Considerations for DNA Amplification
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Quiz #3
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PCR Detection Chemistries SYBR Green
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Quiz #4
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PCR Detection Chemistries Probes
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Quiz #5
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About this course
- Free
- 17 lessons
- 0.5 hours of video content
Meet your instructor
Field Application Specialist David Eaves
David Eaves is an expert in molecular biology techniques, supported by over 15 years of hands-on experience working in both research and clinical environments. He has translated this experience to support researchers as a field application scientist and technical sales specialist at Bio-Rad Laboratories. He also spent 8 years at Cincinnati Children's Hospital Medical Center, serving as a senior molecular pathology technician, supporting clinical sequencing applications.
Reviews for this course
Interesting and precise
David Larrea
A well structured and direct presentation of important information about the basic concepts of RT-qPCR. Very useful and good explanations.
A well structured and direct presentation of important information about the basic concepts of RT-qPCR. Very useful and good explanations.
Read LessGood course for Basic understanding
Anagha Raviprakash
It posed a few challenging questions and is really good for undergrads like myself
It posed a few challenging questions and is really good for undergrads like myself
Read LessFAQ
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Who should take this course?
This course is designed for people who want to have a fundamental understanding of RT-qPCR and gene expression.
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How long will this course take?
This class should take approximately 1 to 1.5 hours to complete.
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Can I get a certificate for this course?
Once all sections of this course have been completed a certificate with your name and course name will be available for download.
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Will I be able to ask questions and get answers?
By enrolling in this class you will be automatically added to Bio-Rad's RT-qPCR and Gene Expression community. This is a highly collaborative place to ask questions and share ideas with the instructors and your peers. You can post pictures, comments and reply to others.
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What are the other classes in this series?
There are five classes in the "Fundamentals of RT-qPCR and Gene Expression" series. Course 1: Introduction to RT-qPCR and gene expression, Course 2: RNA sample preparation considerations, Course 3: Experimental design for gene expression, Course 4: Assay optimization, Course 5: Gene expression analysis
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Do I have to take the classes in order?
It is recommended that you take the 5 fundamentals of RT-qPCR and gene expression classes in order. There are concepts introduced in earlier classes that are referred to in the later classes.
All Courses
In the Fundamentals of RT-qPCR and Gene Expression series