Gene Expression Analysis by RT-qPCR
Course #5 of the Fundamentals of RT-qPCR Series
This is course #5 of a 5-part fundamentals of RT-qPCR series. These courses will give you an understanding of basic RT-qPCR techniques and troubleshooting for each step of the gene expression workflow.
Courses are led by Bio-Rad's Field Application Specialists, who have diligently compiled all the questions they have received in the field and have designed this program to be both comprehensive and efficient.
You will receive a downloadable certificate upon successful completion of each course.
Course Overview
This is a preview, please enroll to access these lessons
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Welcome to the course
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How to use this course
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Meet the instructor
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Pre-Analysis Data Quality Check: Amplification Plots
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Quiz #1
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Pre-Analysis Data Quality Check: Controls and Replicates
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Quiz #2
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Calculating Fold Difference in Gene Expression
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Quiz #3
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Presenting qPCR Data: Plotting, Calculating Error and Determining Significance
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Quiz #4
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Performing Multi-Plate Analysis
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Quiz #5
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About this course
- Free
- 15 lessons
- 0.5 hours of video content
Reviews for this course
Great content!
David Lubertozzi
The tutorials were well paced and very clear, with just the right amount of detail.
The tutorials were well paced and very clear, with just the right amount of detail.
Read LessExcellent
Yarsmin Zeebone
As someone who has never done western blotting before, this course easily gave me a decent idea about what and how western blotting works.
As someone who has never done western blotting before, this course easily gave me a decent idea about what and how western blotting works.
Read LessClear and well presented
Doreen Ugaia
Amazing
Sudhanva D Dixit
It was so good taking this course. This will help me a lot in my career. I would like to thank Bio-Rad for providing such a wonderful opportunity. Thanks a lot.
Read MoreIt was so good taking this course. This will help me a lot in my career. I would like to thank Bio-Rad for providing such a wonderful opportunity. Thanks a lot.
Read Lessinteresting
Ruchira Jayasundara
Excellent fundamentals on Western Blotting
Pauline Parkinson
The course offers a concise informative description of the fundamentals of western blotting; it offers examples and test your knowledge.
The course offers a concise informative description of the fundamentals of western blotting; it offers examples and test your knowledge.
Read LessGreat Western Blotting Course
Zwe WinPaing
This course is very nice for me that I want to understand how many steps are in Western Blotting and what I should consider to get a better result.
This course is very nice for me that I want to understand how many steps are in Western Blotting and what I should consider to get a better result.
Read LessWestern blotting course
Siddhant Jaybhaye
Very nice course by Bio Rad
Very nice course by Bio Rad
Read LessIt's well highlighted
Zibiah Kitur
It has been an eye opener for me to understand the preliminary checks of protein sample before proceeding to load it on the Gel
It has been an eye opener for me to understand the preliminary checks of protein sample before proceeding to load it on the Gel
Read LessMeet your instructor
Field Application Specialist Eric Johnson
Eric Johnson is an accomplished molecular and cellular biologist with a background in neuromuscular disorders, cardiac electrophysiology and transcriptional regulation. Eric received his PhD in Biochemistry from Ohio State University and was formerly a Post Doctorate at Washington University's Department of Cardiology. As a Bio‐Rad Genomics Field Application Scientist, Eric supports researchers in the development and implementation of various genomic techniques including real‐time PCR, droplet digital PCR, and next generation sequencing.
FAQ
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Who should take this course?
This course is designed for people who want to have a fundamental understanding of RT-qPCR and gene expression.
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How long will this course take?
This class should take approximately 1 to 1.5 hours to complete.
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Can I get a certificate for this course?
Once all sections of this course have been completed a certificate with your name and course name will be available for download.
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Will I be able to ask questions and get answers?
By enrolling in this class you will be automatically added to Bio-Rad's RT-qPCR and Gene Expression community. This is a highly collaborative place to ask questions and share ideas with the instructors and your peers. You can post pictures, comments and reply to others.
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What are the other classes in this series?
There are five classes in the "Fundamentals of RT-qPCR and Gene Expression" series. Course 1: Introduction to RT-qPCR and gene expression, Course 2: RNA sample preparation considerations, Course 3: Experimental design for gene expression, Course 4: Assay optimization, Course 5: Gene expression analysis
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Do I have to take the classes in order?
It is recommended that you take the 5 fundamentals of RT-qPCR and gene expression classes in order. There are concepts introduced in earlier classes that are referred to in the later classes.
All Courses
In the Fundamentals of RT-qPCR and Gene Expression series