This is course #3 of a 5-part fundamentals of RT-qPCR series. These courses will give you an understanding of basic RT-qPCR techniques and troubleshooting for each step of the gene expression workflow.


Courses are led by Bio-Rad's Field Application Specialists, who have diligently compiled all the questions they have received in the field and have designed this program to be both comprehensive and efficient.


You will receive a downloadable certificate upon successful completion of each course.

What you will learn

in Course #3: Experimental Design for Gene Expression by RT-qPCR

  • Experimental design considerations

  • Selecting the right controls

  • Biological and technical replicates

  • Considerations for designing primers

  • Considerations for designing probes

Course Overview

This is a preview, please enroll to access these lessons

    1. Welcome to the course

    2. How to use this course

    3. Meet the instructor

    1. Defining the Experimental Aim

    2. Quiz #1

    1. Selecting the Proper Controls

    2. Quiz #2

    1. Biological and Technical Replicates: Best Practices

    2. Quiz #3

    1. Designing Primers and Probes: Best Practices

    2. Quiz #4

    1. Validated Assays

    2. Quiz #5

About this course

  • Free
  • 15 lessons
  • 0.5 hours of video content

Meet your instructor

Field Application Specialist Tara Ellison

Dr. Ellison is a field application scientist with over 20 years of experience in molecular biology and transcriptional regulation in the fields of metabolism, cancer, and biomarker detection. Her current role involves helping researchers develop robust methodologies for ddPCR and qPCR. Dr. Ellison earned a Ph.D. in Pharmacology from Case Western Reserve University and was formerly a postdoctoral fellow at UT Southwestern Medical Center in Dallas.


  • Who should take this course?

    This course is designed for people who want to have a fundamental understanding of RT-qPCR and gene expression.

  • How long will this course take?

    This class should take approximately 1 to 1.5 hours to complete.

  • Can I get a certificate for this course?

    Once all sections of this course have been completed a certificate with your name and course name will be available for download.

  • Will I be able to ask questions and get answers?

    By enrolling in this class you will be automatically added to Bio-Rad's RT-qPCR and Gene Expression community. This is a highly collaborative place to ask questions and share ideas with the instructors and your peers. You can post pictures, comments and reply to others.

  • What are the other classes in this series?

    There are five classes in the "Fundamentals of RT-qPCR and Gene Expression" series. Course 1: Introduction to RT-qPCR and gene expression, Course 2: RNA sample preparation considerations, Course 3: Experimental design for gene expression, Course 4: Assay optimization, Course 5: Gene expression analysis

  • Do I have to take the classes in order?

    It is recommended that you take the 5 fundamentals of RT-qPCR and gene expression classes in order. There are concepts introduced in earlier classes that are referred to in the later classes.