This is course #2 of a 5-part fundamentals of western blotting series. These courses are for anyone who would like to have a detailed understanding of the principles of each step of the western blotting workflow.  

Courses are led by Bio‑Rad’s Field Application Specialists who have diligently compiled all the questions they have received in the field and have designed this program to be both comprehensive and efficient.

You will receive a downloadable certificate upon completion of each course.

What you will learn

in Course #2: Fundamentals of Gel Electrophoresis and Transfer

  • Principles of gel electrophoresis

  • Selecting the best gel for your application

  • Selecting and optimizing the best transfer technique for your application

Learn the fundamentals of gel electrophoresis and protein transfer

Course Overview

This is a preview, please enroll to access these lessons

    1. Welcome to the course

    2. How to use this course

    3. Meet the instructor

    4. Curriculum Overview

    1. Basics of Electrophoresis

    2. Quiz #1

    1. Electrophoresis Staining and Troubleshooting

    2. Quiz #2

    1. Protein Transfer Basics

    2. Quiz #3

    1. Protein Transfer Staining and Troubleshooting

    2. Quiz #4

    1. Gel Electrophoresis and Transfer Resources

    2. Get Your Free Western Blotting Workflow Poster

About this course

  • Free
  • 14 lessons
  • 0.5 hours of video content

Meet your instructor

Proteomics Field Application Specialist Gary Ross

Gary Ross earned his PhD in Biochemistry at the University of Cincinnati School of Medicine in 1984. He worked as a postdoctoral and research associate in the Departments of Neonatology and Pulmonary Biology at Cincinnati Children’s Hospital Medical Center. His research focused on the purification and characterization of pulmonary surfactant proteins. Since joining Bio‑Rad in 2006, he has served as Field Application Specialist supporting SELDI‑MS, ProteOn XPR, and proteomics application technologies.

Reviews for this course

5 star rating

Excellent Fundamentals!

Pauline Parkinson

An excellent fundamental course on gel electrophoresis and transfer. Excellent troubleshooting examples to aid the scientist. I recommend this course for the...

Read More

An excellent fundamental course on gel electrophoresis and transfer. Excellent troubleshooting examples to aid the scientist. I recommend this course for the novice or the expert in gel electrophoresis and transfer.

Read Less
5 star rating

GOOD

Dr Vijayabaskar Pandian

Excellent very use full topics

Excellent very use full topics

Read Less
5 star rating

Awesome

Sudhanva D Dixit

The course is really interesting and awesome. Thank you for providing these courses.

The course is really interesting and awesome. Thank you for providing these courses.

Read Less
5 star rating

curso: Fundamentals of Western Blotting Course #2

BEATRIZ DIAZ SALAS

excelente curso, super didáctico, aprendí mucho en verdad, muchas gracias...

excelente curso, super didáctico, aprendí mucho en verdad, muchas gracias...

Read Less

FAQ

  • Who should take this course?

    This course is designed for people who want to have a fundamental understanding of gel electrophoresis and protein transfer.

  • How long will this course take?

    This class should take approximately 1 to 1.5 hours to complete.

  • Can I get a certificate for this course?

    Once all sections of this course have been completed a certificate with your name and course name will be available for download.

  • Will I be able to ask questions and get answers?

    By enrolling in this class you will be automatically added to Bio-Rad's Western Blotting Community. This is a highly collaborative place to ask questions and share western blotting ideas with the instructors and your peers. You can post pictures, comments and reply to others.

  • What are the other classes in this series?

    There are five classes in the Fundamentals of Western Blotting series. Course 1: Introduction to western blotting & sample prep, Course 2: Gel electrophoresis & transfer, Course 3: Immunodetection of target proteins, Course 4: Image acquisition, Course 5: Image analysis