Fundamentals of Western Blotting Immunodetection
Course #3 of the Fundamentals of Western Blotting Series
This is course #3 of a 5-part fundamentals of western blotting series. These courses are for anyone who would like to have a detailed understanding of the principles of each step of the western blotting workflow.
Courses are led by Bio‑Rad’s Field Application Specialists who have diligently compiled all the questions they have received in the field and have designed this program to be both comprehensive and efficient.
You will receive a downloadable certificate upon completion of each course.
Course Overview
This is a preview, please enroll to access these lessons
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Welcome to the course
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How to use this course
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Meet the instructor
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Immunodetection workflow
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Quiz 1
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Primary Antibody Validation
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Quiz #2
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Primary Antibody Optimization
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Quiz #3
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Secondary Antibody
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Quiz #4
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Linear Dynamic Range of a Western Blot
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Quiz #5
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About this course
- Free
- 19 lessons
- 0.5 hours of video content
Meet your instructor
Field Application Specialist Mauhamad Baarine
Mauhamad Baarine is a Field Application Scientist at Bio-Rad Laboratories. He specializes in Western blotting, Bio-Plex Immunoassays, and Chromatography. Mauhamad completed his postdoctoral fellowship at Medical University of South Carolina in Charleston, SC where he studied the role of mitochondrial HDAC1 activity in myocardial ischemia/reperfusion injury. Mauhamad earned his PhD from the University of Burgundy in France where he developed a new cell line model to study the metabolic dysfunction in X-linked Adrenoleukodystrophy disease.
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Reviews for this course
Excellent fundamental review of immunodetection
Pauline Parkinson
The course was detailed in the principles of immunodetection. An excellent review of the steps is given and troubleshooting is also reviewed. An excellent co...
Read MoreThe course was detailed in the principles of immunodetection. An excellent review of the steps is given and troubleshooting is also reviewed. An excellent course for the novice as well as the expert scientist. I recommend this course for its details and troubleshooting on immunodetection.
Read LessGOOD
Dr Vijayabaskar Pandian
Nice easy to understand
Nice easy to understand
Read LessVery Useful
Joshua Kleam
Opinion about the course
Aamir Sohail
Highly advandaced and validated course makes a researcher able to choose and do the best thing
Highly advandaced and validated course makes a researcher able to choose and do the best thing
Read LessReview
Godfred Yawson Scott
It has a good content
It has a good content
Read LessFAQ
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Who should take this course?
This course is designed for people who want to have a fundamental understanding of gel electrophoresis and protein transfer.
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How long will this course take?
This class should take approximately 1 to 1.5 hours to complete.
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Can I get a certificate for this course?
Once all sections of this course have been completed a certificate with your name and course name will be available for download.
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Will I be able to ask questions and get answers?
By enrolling in this class you will be automatically added to Bio-Rad's Western Blotting Community. This is a highly collaborative place to ask questions and share western blotting ideas with the instructors and your peers. You can post pictures, comments and reply to others.
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What are the other classes in this series?
There are five classes in the Fundamentals of Western Blotting series. Course 1: Introduction to western blotting & sample prep, Course 2: Gel electrophoresis & transfer, Course 3: Immunodetection of target proteins, Course 4: Image acquisition, Course 5: Image analysis
All Courses
In the fundamentals of western blotting series