Fundamentals of Western Blotting Sample Preparation

Fundamentals of Western Blotting and Sample Preparation

Course #1 of the Fundamentals of Western Blotting Series

Enroll for free

This is course #1 of a 5-part fundamentals of western blotting series. These courses are for anyone who would like to have a detailed understanding of the principles of each step of the western blotting workflow.

Courses are led by Bio‑Rad’s Field Application Specialists who have diligently compiled all the questions they have received in the field and have designed this program to be both comprehensive and efficient.

You will receive a downloadable certificate upon completion of each course.

What you will learn

in Course #1: Fundamentals of Western Blotting and Sample Preparation

How western blots are used in biological research
Experimental design considerations before you begin your project
Reagents and methods for good sample preparation

Learn the fundamentals of western blotting and sample preparation

Enroll for free

Course Overview

This is a preview, please enroll to access these lessons

1. Welcome to the course
2. How to use this course
3. Meet the instructor
4. Survey: What are the challenges you have with western blotting?
1. Introduction to Protein Detection
2. Quiz #1
1. Western Blot Overview
2. Quiz #2
1. Introduction to Sample Prep & Lysis Methods
2. Quiz #3
1. Solubilization, Stabilization & Enrichment
2. Quiz #4
1. Sample Cleanup, Quantitation & Preparation for PAGE
2. Quiz #5
1. Sample Preparation Resources
2. Get Your Free Western Blotting Workflow Poster

About this course

Free
16 lessons
0.5 hours of video content

Reviews for this course

Great content!

David Lubertozzi

The tutorials were well paced and very clear, with just the right amount of detail.

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Description about the course

Girija Prasanna Sahoo

This course will give you knowledge about fundamentals of western blotting. After completion of this course you will able to know what are the steps involves...

This course will give you knowledge about fundamentals of western blotting. After completion of this course you will able to know what are the steps involves along with the reagents and their functions.

Read Less

Great introductory course

Godwin Woode

Tutor explains course very well and in an orderly manner.

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Western Blotting Course

Rafat Hossain Rafi

It reminded the academic class for learning western blotting. Great Course & Great Initiatives Thank you instructor

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Interesante técnica molecular

Eduardo Barroso

Agradezco por tan genios a información sobre la técnica molecular wastern blots, me de Ayuda entender cada paso y proceso y el producto a obtener para la lín...

Agradezco por tan genios a información sobre la técnica molecular wastern blots, me de Ayuda entender cada paso y proceso y el producto a obtener para la línea de investigación a realizar precisando la proteína de interés en los inmunoensayos excelente profesor didáctico y preciso felicidades

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Course

PANKAJ LADWAL

short, crisp, to the point, clarity, skills enhancer

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Great course

Juan Molina

An excellent introductory course to the technic, thank you very much!

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Excellent course

Sherin James

I would encourage every science graduate student to take this course.

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Meet your instructor

Proteomics Field Application Specialist Paul Pease

Paul Pease is currently a Field Application Specialist for Bio‑Rad Laboratories in the Central Plains Region of the United States. He joined Bio‑Rad in 2015 and specializes in Western Blotting, Chromatography and Bio‑Plex Multiplex ELISA applications. He earned his PhD from the University of California, Berkeley where he used single‑molecule methods to study the biophysics of DNA motor proteins. His postdoctoral work at the University of California, Davis utilized single‑molecule fluorescence microscopy to investigate DNA repair mechanisms.

FAQ

Who should take this course?

This course is designed for people who want to have a fundamental understanding of western blotting and sample preparation.
How long will this course take?

This class should take approximately 1 to 1.5 hours to complete.
Can I get a certificate for this course?

Once all sections of this course have been completed a certificate with your name and course name will be available for download.
Will I be able to ask questions and get answers?

By enrolling in this class you will be automatically added to Bio-Rad's Western Blotting Community. This is a highly collaborative place to ask questions and share western blotting ideas with the instructors and your peers. You can post pictures, comments and reply to others.
What are the other classes in this series?

There are five classes in the "Fundamentals of Western Blotting" series. Course 1: Introduction to western blotting & sample prep, Course 2: Gel electrophoresis & transfer, Course 3: Immunodetection of target proteins, Course 4: Image acquisition, Course 5: Image analysis
Do I have to take the classes in order?

It is recommended that you take the 5 fundamentals of western blotting classes in order. There are concepts introduced in earlier classes that are referred to in the later classes.