Advanced Western Blotting
Course 1: Sample Preparation
Now that you've taken the 5-part fundamentals of western blotting series, you are ready to begin section 1 of this 6-part advanced course series.
These advanced western blotting courses provide more comprehensive information on the topics that were first discussed in the fundamentals courses.
In this course, you will learn about these sample preparation topics: protein sources, cell lysis, sample preparation buffers, and reduction. More sections will be added to this course soon, and you will be notified via email as they become available.
Courses are led by Bio‑Rad’s Western Blotting Specialists who have years of collective lab, teaching, and customer troubleshooting experience.
You will receive a downloadable certificate and a western blotting poster upon completion of the course.
Course Overview
This is a preview, please enroll to access these lessons
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Welcome to the course
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How to use this course
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Meet the instructors
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Sample Preparation Overview
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Quiz #1 - Sample Preparation Overview
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Protein Sources Part 1 - Introduction
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Protein Sources Part 2 - Cell Derived
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Quiz #2 - Cell Derived Protein Sources
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Protein Sources Part 3 - Secretome
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Quiz #3 - Secretome
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Protein Sources Part 4 - Biological Fluid
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Quiz #4 - Biological Fluid
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Cell Lysis Methods Part 1 (Mechanical)
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Quiz #5 Cell Lysis Methods (Mechanical)
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Cell Lysis Methods Part 1 (Non-Mechanical)
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Quiz #6 Cell Lysis Methods (Non-Mechanical)
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Sample Preparation Buffers (Part 1)
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Quiz #7 - Sample Preparation Buffers (Part 1)
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Sample Preparation Buffers (Part 2)
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Quiz #8 - Sample Preparation Buffers (Part 2)
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Reduction
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Quiz #9 - Reduction
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About this course
- Free
- 24 lessons
- 2.5 hours of video content
Meet your instructors
Matthew Hammond
Matt has been with Bio-Rad Laboratories since 2010, and spent most of that time in a dual role as a product development scientist and an applications scientist working on western blot imagers and consumables that span the western blotting workflow. He has published technical documents, from white papers to peer-reviewed articles, on the topic of validating western blots. He has also provided technical support and training for both the Bio-Rad sales force and customers. He received his Ph.D. in Analytical Chemistry at Stanford University.
Lindsey Maccoux
Lindsey has been with Bio-Rad since 2016, where she has held positions in Sales and Marketing. At Bio-Rad, she has managed products in Chromatography, qPCR, and now Western Blotting. Prior to Bio-Rad she did a Post-Doc in Biomedical Engineering at the University of Wisconsin, Madison and held roles in Product Development and Sales at biotech start-ups in Madison. She received her Master’s in Immunology and Immunogenetics from the University of Manchester, England and a Ph.D. in Cancer Biology and Biomedical Engineering at the Technische Universität Dortmund, Germany. Her previous research involved studying signaling pathways in HER2+ breast cancer to develop new microfluidic techniques to better understand the tumor microenvironment.
Sahel Mohebbi
Sahel earned her Ph.D. in Bioinorganic chemistry from the University of Georgia, where she focused on the spectroscopic characterization of metalloenzymes and biological iron-sulfur centers. She unraveled a unique function for a novel [Fe-S] cluster storage protein and developed a new pathway for [Fe-S] cluster assembly on recipient proteins. Upon graduation, she joined an immunology and cancer biology research group at the University of Texas MD Anderson Cancer Center, focusing on the effect of diet and micro-nutrition on immune response, gut microbiota, and liver cancer development. She represents Bio-Rad as the Proteomics Field Application Scientist specializing in Western Blotting, Bio-Plex Immunoassays, and Chromatography.
Deanna Woo
Deanna joined Bio-Rad in 2013 as a Research Associate, where she validated antibodies for the PrecisionAb Antibodies product line, becoming an expert in Western Blotting. She was soon promoted to Application Scientist, where she became familiar with numerous Bio-Rad product lines used in the study of proteomics, molecular biology, and genomics. She received her Bachelor of Science at San Francisco State University in Cellular and Molecular Biology, with a minor in Chemistry, and held research roles at UCSF, Genentech, and Stanford. Her previous research work included studying the molecular mechanisms that regulate the development of primordial germ cells and research exploring hyperphosphorylation of tau in neurodegenerative diseases.
Kenneth Oh
Kenneth earned his Ph.D. in bioorganic chemistry at UC Santa Barbara, where he synthesized fluorescence‑based peptide beacons that measured nucleic acid and protein binding events. Upon graduation, he co‑founded a biotech company that re‑engineered virus capsids that served as a drug delivery platform. At Bio‑Rad, he manages the electrophoresis and western blotting product portfolio.
FAQ
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Who should take this course?
This course provides a deep dive into concepts related to western blotting and is recommended for anyone interested in learning about advanced western blot concepts and techniques.
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Is there a prerequisite for the advanced courses?
A basic understanding of western blotting or it is recommended that you take the 5 fundamentals of western blotting courses before beginning these advanced courses. There are concepts introduced in the fundamental courses that are referred to in the advanced courses.
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How long will this course take?
This course should take approximately 3 - 4 hours to complete.
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Can I get a certificate for this course?
Once all sections of this course have been completed a certificate with your name and course name will be available for download.
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Will I be able to ask questions and get answers?
By enrolling in this course you will be automatically added to Bio-Rad's Western Blotting Community. This is a highly collaborative place to ask questions and share western blotting ideas with the instructors and your peers. You can post pictures, comments and reply to others.
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What are the other courses in this series?
There will be 6 courses in the Advanced Western Blotting Series. Course 1: Sample Preparation, Course 2: Electrophoresis, Course 3: Transfer, Course 4: Immunodetection, Course 5: Image Acquisition, and Course 6: Image Analysis.
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When will the other advanced western blotting courses become available?
Our western blotting specialists are continuously adding new content to this advanced series. Stay tuned for the next section!
All Courses in the Western Blotting Series
Advanced Western Blotting Courses:
Course 2: Electrophoresis - coming soon
Course 3: Transfer - coming soon
Course 4: Immunodetection - coming soon
Course 5: Image Acquisition - coming soon
Course 6: Image Analysis - coming soon
Fundamental Western Blotting Courses:
Course 1: Western Blotting Overview & Sample Preparation