Now that you've taken the 5-part fundamentals of western blotting series, you are ready to begin section 1 of this 6-part advanced course series.

These advanced western blotting courses provide more comprehensive information on the topics that were first discussed in the fundamentals courses. 

In this course, you will learn about these sample preparation topics: protein sources, cell lysis, sample preparation buffers, and reduction. More sections will be added to this course soon, and you will be notified via email as they become available.

Courses are led by Bio‑Rad’s Western Blotting Specialists who have years of collective lab, teaching, and customer troubleshooting experience.

You will receive a downloadable certificate and a western blotting poster upon completion of the course.

What you will learn

in Advanced Course 1: Sample Preparation

  • Experimental design considerations before you begin your project

  • Reagents and methods for good sample preparation

  • Review western blotting case studies

Course Overview

This is a preview, please enroll to access these lessons

    1. Welcome to the course

    2. How to use this course

    3. Meet the instructors

    1. Sample Preparation Overview

    2. Quiz #1 - Sample Preparation Overview

    1. Protein Sources Part 1 - Introduction

    2. Protein Sources Part 2 - Cell Derived

    3. Quiz #2 - Cell Derived Protein Sources

    4. Protein Sources Part 3 - Secretome

    5. Quiz #3 - Secretome

    6. Protein Sources Part 4 - Biological Fluid

    7. Quiz #4 - Biological Fluid

    1. Cell Lysis Methods Part 1 (Mechanical)

    2. Quiz #5 Cell Lysis Methods (Mechanical)

    3. Cell Lysis Methods Part 1 (Non-Mechanical)

    4. Quiz #6 Cell Lysis Methods (Non-Mechanical)

    1. Sample Preparation Buffers (Part 1)

    2. Quiz #7 - Sample Preparation Buffers (Part 1)

    3. Sample Preparation Buffers (Part 2)

    4. Quiz #8 - Sample Preparation Buffers (Part 2)

    1. Reduction

    2. Quiz #9 - Reduction

About this course

  • Free
  • 24 lessons
  • 2.5 hours of video content

Meet your instructors

Course Reviews

FAQ

  • Who should take this course?

    This course provides a deep dive into concepts related to western blotting and is recommended for anyone interested in learning about advanced western blot concepts and techniques.

  • Is there a prerequisite for the advanced courses?

    A basic understanding of western blotting or it is recommended that you take the 5 fundamentals of western blotting courses before beginning these advanced courses. There are concepts introduced in the fundamental courses that are referred to in the advanced courses.

  • How long will this course take?

    This course should take approximately 3 - 4 hours to complete.

  • Can I get a certificate for this course?

    Once all sections of this course have been completed a certificate with your name and course name will be available for download.

  • Will I be able to ask questions and get answers?

    By enrolling in this course you will be automatically added to Bio-Rad's Western Blotting Community. This is a highly collaborative place to ask questions and share western blotting ideas with the instructors and your peers. You can post pictures, comments and reply to others.

  • What are the other courses in this series?

    There will be 6 courses in the Advanced Western Blotting Series. Course 1: Sample Preparation, Course 2: Electrophoresis, Course 3: Transfer, Course 4: Immunodetection, Course 5: Image Acquisition, and Course 6: Image Analysis.

  • When will the other advanced western blotting courses become available?

    Our western blotting specialists are continuously adding new content to this advanced series. Stay tuned for the next section!

All Courses in the Western Blotting Series

Advanced Western Blotting Courses:

Course 1: Sample Preparation

Course 2: Electrophoresis - coming soon

Course 3: Transfer - coming soon 

Course 4: Immunodetection - coming soon 

Course 5: Image Acquisition - coming soon 

Course 6: Image Analysis - coming soon 


Fundamental Western Blotting Courses:

Course 1: Western Blotting Overview & Sample Preparation

Course 2: Gel Electrophoresis and Transfer

Course 3: Immunodetection

Course 4: Image Acquisition

Course 5: Image Analysis