Advanced Western Blotting
Course 1: Sample Preparation
Now that you've taken the 5-part fundamentals of western blotting series, you are ready to begin section 1 of this 6-part advanced course series.
These advanced western blotting courses provide more comprehensive information on the topics that were first discussed in the fundamentals courses.
In this course, you will learn about these sample preparation topics: protein sources, cell lysis, sample preparation buffers, and reduction. More sections will be added to this course soon, and you will be notified via email as they become available.
Courses are led by Bio‑Rad’s Western Blotting Specialists who have years of collective lab, teaching, and customer troubleshooting experience.
You will receive a downloadable certificate and a western blotting poster upon completion of the course.
Deepen your western blotting sample preparation knowledge
This is a preview, please enroll to access these lessons
Welcome to the course
How to use this course
Meet the instructors
Sample Preparation Overview
Quiz #1 - Sample Preparation Overview
Protein Sources Part 1 - Introduction
Protein Sources Part 2 - Cell Derived
Quiz #2 - Cell Derived Protein Sources
Protein Sources Part 3 - Secretome
Quiz #3 - Secretome
Protein Sources Part 4 - Biological Fluid
Quiz #4 - Biological Fluid
Cell Lysis Methods Part 1 (Mechanical)
Quiz #5 Cell Lysis Methods (Mechanical)
Cell Lysis Methods Part 1 (Non-Mechanical)
Quiz #6 Cell Lysis Methods (Non-Mechanical)
Sample Preparation Buffers (Part 1)
Quiz #7 - Sample Preparation Buffers (Part 1)
Sample Preparation Buffers (Part 2)
Quiz #8 - Sample Preparation Buffers (Part 2)
Quiz #9 - Reduction
This course provides a deep dive into concepts related to western blotting and is recommended for anyone interested in learning about advanced western blot concepts and techniques.
A basic understanding of western blotting or it is recommended that you take the 5 fundamentals of western blotting courses before beginning these advanced courses. There are concepts introduced in the fundamental courses that are referred to in the advanced courses.
This course should take approximately 3 - 4 hours to complete.
Once all sections of this course have been completed a certificate with your name and course name will be available for download.
By enrolling in this course you will be automatically added to Bio-Rad's Western Blotting Community. This is a highly collaborative place to ask questions and share western blotting ideas with the instructors and your peers. You can post pictures, comments and reply to others.
There will be 6 courses in the Advanced Western Blotting Series. Course 1: Sample Preparation, Course 2: Electrophoresis, Course 3: Transfer, Course 4: Immunodetection, Course 5: Image Acquisition, and Course 6: Image Analysis.
Our western blotting specialists are continuously adding new content to this advanced series. Stay tuned for the next section!
Advanced Western Blotting Courses:
Course 2: Electrophoresis - coming soon
Course 3: Transfer - coming soon
Course 4: Immunodetection - coming soon
Course 5: Image Acquisition - coming soon
Course 6: Image Analysis - coming soon
Fundamental Western Blotting Courses:
Course 1: Western Blotting Overview & Sample Preparation
Course 2: Gel Electrophoresis and Transfer