This is course #2 of a 5-part fundamentals of RT-qPCR series. These courses will give you an understanding of basic RT-qPCR techniques and troubleshooting for each step of the gene expression workflow.


Courses are led by Bio-Rad's Field Application Specialists, who have diligently compiled all the questions they have received in the field and have designed this program to be both comprehensive and efficient.


You will receive a downloadable certificate upon successful completion of each course.

What you will learn

in Course #2: RNA Sample Preparation Considerations for RT‑qPCR

  • Overview of RNA function, diversity, and key characteristics

  • RNA Isolation: Methods and precautions to ensure a pure and intact RNA

  • cDNA Synthesis: Review RT process, enzymes, and suitability for different RNA biotypes

  • RNA quantification/quality methods

Course Overview

This is a preview, please enroll to access these lessons

    1. Welcome to the course

    2. How to use this course

    3. Meet the instructor

    1. RNA Function, Diversity, and Key Characteristics

    2. Quiz #1

    1. RNA Isolation: The Importance of Pure, Intact Extract

    2. Quiz #2

    1. Exploring Methods to Validate Extract Quality and Quantity

    2. Quiz #3

    1. cDNA Synthesis: RT Process, Enzymes, and Suitability for RNA Biotypes

    2. Quiz #4

    1. Sample Preparation for RT-qPCR Resources

    2. Get Your Free Gene Expression University Poster

About this course

  • Free
  • 13 lessons
  • 1 hour of video content

Meet your instructor

Field Application Specialist Jordan Sheets

Jordan Sheets represents Bio‑Rad as the Genomics Field Application Scientist (GFAS) for the western region of America. His current focus includes consulting pharmaceutical companies to assist in the development of new and current therapies that rely heavily on genomics techniques, such as real‑time PCR (qPCR), droplet digital PCR (ddPCR), and next generation sequencing. Jordan earned his Master’s degree in Integrative Genomics from Black Hills State University (BHSU) in 2012. Shortly thereafter, Jordan enrolled in the Basic Biomedical Sciences (BBS) program through the University of South Dakota Sanford School of Medicine (USDSSM), completing his PhD in 2017, followed by a 2‑year postdoctoral training program at Sanford Research (2017‑2019). During his education and training, Jordan has acquired valuable experience managing projects surrounding SARS‑CoV‑2 detection, cancer biology, neuroscience, stem cell biology, and ecological evolution.

Reviews for this course

5 star rating

So interesting

Syahruni Hidayatullah

The material simple and easy to understand

The material simple and easy to understand

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5 star rating

RNA preparation

Raphael Nyaruaba

The topic is well-explained and helpful. Kudos to the instructor

The topic is well-explained and helpful. Kudos to the instructor

Read Less
5 star rating


David Larrea

Good to refresh concepts and learn basic but utterly important aspects of Sample Preparation and the RT reaction.

Good to refresh concepts and learn basic but utterly important aspects of Sample Preparation and the RT reaction.

Read Less
5 star rating


Pravesh Kumar Gautam

good learning experience

good learning experience

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  • Who should take this course?

    This course is designed for people who want to have a fundamental understanding of RT-qPCR and gene expression.

  • How long will this course take?

    This class should take approximately 1 to 1.5 hours to complete.

  • Can I get a certificate for this course?

    Once all sections of this course have been completed a certificate with your name and course name will be available for download.

  • Will I be able to ask questions and get answers?

    By enrolling in this class you will be automatically added to Bio-Rad's RT-qPCR and Gene Expression community. This is a highly collaborative place to ask questions and share ideas with the instructors and your peers. You can post pictures, comments and reply to others.

  • What are the other classes in this series?

    There are five classes in the "Fundamentals of RT-qPCR and Gene Expression" series. Course 1: Introduction to RT-qPCR and gene expression, Course 2: RNA sample preparation considerations, Course 3: Experimental design for gene expression, Course 4: Assay optimization, Course 5: Gene expression analysis

  • Do I have to take the classes in order?

    It is recommended that you take the 5 fundamentals of RT-qPCR and gene expression classes in order. There are concepts introduced in earlier classes that are referred to in the later classes.