This is course 7 of a 10-part Fundamentals of Flow Cytometry series. It is aimed at anyone who would like to have a detailed understanding of the basics of flow cytometry and stay ahead in all experiments.    

Courses are led by Bio‑Rad's flow cytometry experts who have diligently compiled all the questions they are regularly asked about the application and have designed this program to be both comprehensive and efficient.

You will receive a downloadable certificate upon completion of each course.

What you will learn

Course 7: Successful Multicolor Flow Cytometry

  • How to choose compatible fluorescent dyes

  • What tandem dyes are and considerations for using them

  • Essential controls you should include when building multicolor panels

Successful Muticolor Flow Cytometry

Best practices for building panels

Course Overview

This is a preview, please enroll to access these lessons

    1. Welcome to the course

    2. How to use this course

    3. Meet the instructor

    1. Fluorescent Dye Selection

    2. Chapter 1 Quiz

    1. Example of Multicolor Panel Building

    2. Chapter 2 Quiz

    1. Useful Guides and Tools

About this course

  • Free
  • 8 lessons

Meet your instructor

Flow Cytometry Account Executive Katherine Pline

Katherine graduated from Queen Mary University of London with a BSc (Hons.) in Genetics. She then moved to the University of Sheffield where she completed her thesis on host-pathogen interactions and macrophage polarization in Cryptococcus neoformans fungal infection. She then moved to NYC to continue her academic career investigating the role of chronic viral infection in the induction of lymphomagenesis before joining Bio-Rad as a flow cytometry field application specialist. She is now an account executive on the West Coast in Southern California.


  • Who should take this course?

    This course is designed for people who want to have a fundamental understanding of how flow cytometry works and how it can be used to answer research questions.

  • How long will this course take?

    This class should take approximately 40 minutes to complete.

  • Can I get a certificate for this course?

    Once all sections of this course have been completed a certificate with your name and course name will be available for download.

  • Will I be able to ask questions and get answers?

    By enrolling in this class you will be automatically added to Bio-Rad's Flow Cytometry Community. This is a highly collaborative place to ask questions and share flow cytometry ideas with the instructors and your peers. You can post pictures, comments, and reply to others.

  • What are the other classes in this series?

    There are ten classes in the Fundamentals of Flow Cytometry Series. Course 1: Flow cytometry basics. Course 2: Inside a flow cytometer. Course 3: Fluorescence and flow cytometry principles. Course 4: Sample preparation essentials. Course 5: Target protein detection. Course 6: How to use controls. Course 7: Successful multicolor flow cytometry. Course 8: Data acquisition and analysis. Course 9: Cell sorting essentials. Course 10: Planning flow cytometry experiments.