This is course 2 of a 10-part fundamentals of flow cytometry series. It is aimed at anyone who would like to have a detailed understanding of the basics of flow cytometry and stay ahead in all experiments.    

Courses are led by Bio‑Rad's flow cytometry experts who have diligently compiled all the questions they are regularly asked about the application and have designed this program to be both comprehensive and efficient.

You will receive a downloadable certificate upon completion of each course.

What you will learn

in Course 2: Inside a Flow Cytometer

  • The three main systems of a flow cytometer

  • How the systems interact to deliver functional flow cytometry

  • How laser and filter combinations relate to fluorophore choices

Course Overview

This is a preview, please enroll to access these lessons

    1. Welcome to the course

    2. How to use this course

    3. Meet the instructor

    1. The Fluidics System

    2. Chapter 1 Quiz

    1. The Optics System

    2. Chapter 2 Quiz

    1. Chapter III: The Electronics System

    2. Chapter 3 Quiz

    1. Summary of the Inner Workings of a Flow Cytometer

    2. Chapter 4 Quiz

    1. Useful Guides and Tools

About this course

  • Free
  • 12 lessons

Meet your instructor

Technical Editor Flow Cytometry and Antibodies Chloe Fenton

Your instructor for this course is Chloe Fenton, PhD. Chloe graduated from the University of Manchester, UK, with a degree in Biomedical Sciences. She then carried out her Master’s in immunology at Newcastle University, UK, before moving to the University of Birmingham, UK, to complete her PhD thesis investigating the role of pre-receptor steroid metabolism in the anti-inflammatory effects of therapeutic glucocorticoids. In 2020, she moved to the University of Wuerzburg, Germany, for an immunology postdoctoral position focusing on how regulatory T cells suppress CD8 T cell function during viral infections. Chloe joined Bio-Rad in 2023 as a Technical Editor in Flow Cytometry and Antibodies.


  • Who should take this course?

    This course is designed for people who want to have a fundamental understanding of how flow cytometry works and how it can be used to answer research questions.

  • How long will this course take?

    This class should take approximately 35 minutes to complete.

  • Can I get a certificate for this course?

    Once all sections of this course have been completed a certificate with your name and course name will be available for download.

  • Will I be able to ask questions and get answers?

    By enrolling in this class you will be automatically added to Bio-Rad's Flow Cytometry Community. This is a highly collaborative place to ask questions and share flow cytometry ideas with the instructors and your peers. You can post pictures, comments, and reply to others.

  • What are the other classes in this series?

    There are ten classes in the Fundamentals of Flow Cytometry Series. Course 1: Flow cytometry basics Course 2: Inside a flow cytometer, Course 3: Fluorescence and flow cytometry principles, Course 4: Sample preparation essentials, Course 5: Target protein detection, Course 6: How to use controls, Course 7: Successful multicolor flow cytometry, Course 8: Data Acquisition and Analysis, Course 9: Cell sorting essentials, Course 10: Planning flow cytometry experiments.