Fundamentals of Flow Cytometry Course 5: Target Detection

Target Protein Detection

Course 5 of the Fundamentals of Flow Cytometry Series

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This is course 5 of a 10-part Fundamentals of Flow Cytometry series. It is aimed at anyone who would like to have a detailed understanding of the basics of flow cytometry and stay ahead in all experiments.

Courses are led by Bio‑Rad's flow cytometry experts who have diligently compiled all the questions they are regularly asked about the application and have designed this program to be both comprehensive and efficient.

You will receive a downloadable certificate upon completion of each course.

What you will learn

Course 5: Target Protein Detection

The different methods for studying specific proteins
How to choose a suitable antibody
Why you should titrate your antibody

Learn how to detect your target protein

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Course Overview

This is a preview, please enroll to access these lessons

1. Welcome to the course
2. How to use this course
3. Meet the instructor
1. Essential Principles of Target Detection
2. Chapter 1 Quiz
1. Working with Antibodies
2. Chapter 2 Quiz
1. Useful Guides and Tools

About this course

Free
8 lessons

Meet your instructor

Research Associate Flow Cytometry Anita Wong

Your instructor for this course is Anita Wong. Anita earned her Master’s degree in Advanced Pharmaceutical Manufacturing from the University of Strathclyde where she also obtained her undergraduate degree in Biochemistry and Immunology. She worked for William Grant and Sons (distillery) in Glasgow as a Lab Assistant and acquired valuable laboratory skills before moving to Oxford to work for Bio-Rad. Anita is currently working at Bio-Rad as an Application Scientist. She joined Bio-Rad in 2017 where she worked in Quality Control and throughout her time here has gained good knowledge of ELISA, western blotting, flow cytometry, and immunohistochemistry

FAQ

Who should take this course?

This course is designed for people who want to have a fundamental understanding of how flow cytometry works and how it can be used to answer research questions.
How long will this course take?

This class should take approximately 35 minutes to complete.
Can I get a certificate for this course?

Once all sections of this course have been completed a certificate with your name and course name will be available for download.
Will I be able to ask questions and get answers?

By enrolling in this class you will be automatically added to Bio-Rad's Flow Cytometry Community. This is a highly collaborative place to ask questions and share flow cytometry ideas with the instructors and your peers. You can post pictures, comments, and reply to others.
What are the other classes in this series?

There are ten classes in the Fundamentals of Flow Cytometry Series. Course 1: Flow cytometry basics. Course 2: Inside a flow cytometer. Course 3: Fluorescence and flow cytometry principles. Course 4: Sample preparation essentials. Course 5: Target protein detection. Course 6: How to use controls. Course 7: Successful multicolor flow cytometry. Course 8: Data acquisition and analysis. Course 9: Cell sorting essentials, Course 10: Planning flow cytometry experiments.