This is course 6 of a 10-part Fundamentals of Flow Cytometry series. It is aimed at anyone who would like to have a detailed understanding of the basics of flow cytometry and stay ahead in all experiments.    

Courses are led by Bio‑Rad's flow cytometry experts who have diligently compiled all the questions they are regularly asked about the application and have designed this program to be both comprehensive and efficient.

You will receive a downloadable certificate upon completion of each course.

What you will learn

Course 6: How to Use Controls

  • What are commonly used controls for flow cytometry

  • When you should use isotype controls

  • How to use compensation controls

Course Overview

This is a preview, please enroll to access these lessons

    1. Welcome to the course

    2. How to use this course

    3. Meet the instructor

    1. General Controls

    2. Chapter 1 Quiz

    1. Isotype Controls

    2. Chapter 2 Quiz

    1. Compensation Controls

    2. Chapter 3 Quiz

    1. Useful Guides and Tools

About this course

  • Free
  • 10 lessons

Meet your instructor

Flow Cytometry Specialist Michelle Campbell

Your instructor for this course is Michelle Campbell, PhD. Michelle graduated from the University of the West of Scotland, UK, with a degree in Applied Biosciences & Microbiology, and then continued at the University of the West of Scotland to pursue a PhD in Immunology in collaboration with the University of Glasgow, UK, with her thesis focusing on B cells in rheumatoid arthritis. Michelle then moved to the University of Manchester, UK, to work on mast cells in skin immunobiology, before moving to the University of Leeds, UK, to return to B cell and plasma cell research in drug discovery. Michelle left academic research in 2019 to join the world of clinical trials as a Flow Cytometry Principal Scientist at a Global Central Lab. Michelle joined Bio-Rad in 2022 as a Regional Flow Cytometry Specialist, providing application and technical support across the UK, Nordics, and Eastern Europe.


  • Who should take this course?

    This course is designed for people who want to have a fundamental understanding of how flow cytometry works and how it can be used to answer research questions.

  • How long will this course take?

    This class should take approximately 50 minutes to complete.

  • Can I get a certificate for this course?

    Once all sections of this course have been completed a certificate with your name and course name will be available for download.

  • Will I be able to ask questions and get answers?

    By enrolling in this class you will be automatically added to Bio-Rad's Flow Cytometry Community. This is a highly collaborative place to ask questions and share flow cytometry ideas with the instructors and your peers. You can post pictures, comments, and reply to others.

  • What are the other classes in this series?

    There are ten classes in the Fundamentals of Flow Cytometry Series. Course 1: Flow cytometry basics. Course 2: Inside a flow cytometer. Course 3: Fluorescence and flow cytometry principles. Course 4: Sample preparation essentials. Course 5: Target protein detection. Course 6: How to use controls. Course 7: Successful multicolor flow cytometry. Course 8: Data acquisition and analysis. Course 9: Cell sorting essentials. Course 10: Planning flow cytometry experiments.

All Courses

In the fundamentals of flow cytometry series