Fundamentals of Western Blotting and Sample Preparation
Course #1 of the Fundamentals of Western Blotting Series
This is course #1 of a 5-part fundamentals of western blotting series. These courses are for anyone who would like to have a detailed understanding of the principles of each step of the western blotting workflow.
Courses are led by Bio‑Rad’s Field Application Specialists who have diligently compiled all the questions they have received in the field and have designed this program to be both comprehensive and efficient.
You will receive a downloadable certificate upon completion of each course.
This is a preview, please enroll to access these lessons
Welcome to the course
How to use this course
Meet the instructor
Survey: What are the challenges you have with western blotting?
Introduction to Protein Detection
Quiz #1
Western Blot Overview
Quiz #2
Introduction to Sample Prep & Lysis Methods
Quiz #3
Solubilization, Stabilization & Enrichment
Quiz #4
Sample Cleanup, Quantitation & Preparation for PAGE
Quiz #5
The tutorials were well paced and very clear, with just the right amount of detail.
The tutorials were well paced and very clear, with just the right amount of detail.
Read LessAs someone who has never done western blotting before, this course easily gave me a decent idea about what and how western blotting works.
As someone who has never done western blotting before, this course easily gave me a decent idea about what and how western blotting works.
Read LessIt was so good taking this course. This will help me a lot in my career. I would like to thank Bio-Rad for providing such a wonderful opportunity. Thanks a lot.
Read MoreIt was so good taking this course. This will help me a lot in my career. I would like to thank Bio-Rad for providing such a wonderful opportunity. Thanks a lot.
Read LessThe course offers a concise informative description of the fundamentals of western blotting; it offers examples and test your knowledge.
The course offers a concise informative description of the fundamentals of western blotting; it offers examples and test your knowledge.
Read LessThis course is very nice for me that I want to understand how many steps are in Western Blotting and what I should consider to get a better result.
This course is very nice for me that I want to understand how many steps are in Western Blotting and what I should consider to get a better result.
Read LessVery nice course by Bio Rad
Very nice course by Bio Rad
Read LessIt has been an eye opener for me to understand the preliminary checks of protein sample before proceeding to load it on the Gel
It has been an eye opener for me to understand the preliminary checks of protein sample before proceeding to load it on the Gel
Read LessThis course is designed for people who want to have a fundamental understanding of western blotting and sample preparation.
This class should take approximately 1 to 1.5 hours to complete.
Once all sections of this course have been completed a certificate with your name and course name will be available for download.
By enrolling in this class you will be automatically added to Bio-Rad's Western Blotting Community. This is a highly collaborative place to ask questions and share western blotting ideas with the instructors and your peers. You can post pictures, comments and reply to others.
There are five classes in the "Fundamentals of Western Blotting" series. Course 1: Introduction to western blotting & sample prep, Course 2: Gel electrophoresis & transfer, Course 3: Immunodetection of target proteins, Course 4: Image acquisition, Course 5: Image analysis
It is recommended that you take the 5 fundamentals of western blotting classes in order. There are concepts introduced in earlier classes that are referred to in the later classes.
In the fundamentals of western blotting series