This is course #5 of a 5-part fundamentals of western blotting series. These courses are for anyone who would like to have a detailed understanding of the principles of each step of the western blotting workflow.  

Courses are led by Bio‑Rad’s Field Application Specialists who have diligently compiled all the questions they have received in the field and have designed this program to be both comprehensive and efficient.

You will receive a downloadable certificate upon completion of each course.

What you will learn

in Course #5: Fundamentals of Western Blotting Image Analysis

  • Removing background from your data

  • How to quantify your target protein

  • Normalizing your data to controls

  • Calculating relative expression levels of your target protein

Learn the fundamentals of Western Blotting Image Analysis

Enroll for free

Course Overview

This is a preview, please enroll to access these lessons

    1. Welcome to the course

    2. How to use this course

    3. Meet the instructor

    1. Image Analysis Overview

    2. Quiz #1

    1. Identify Bands & Background Subtraction (Part 1)

    2. Quiz #2

    1. Background Subtraction (Part 2)

    2. Quiz #3

    1. Normalize to Loading Control

    2. Quiz #4

    1. Calculate Relative Abundance

    2. Quiz #5

About this course

  • Free
  • 15 lessons
  • 0.5 hours of video content

Meet your instructor

Global Marketing Manager Kenneth Oh

Kenneth Oh earned his PhD in bioorganic chemistry at UC Santa Barbara, where he synthesized fluorescence‑based peptide beacons that measured nucleic acid and protein binding events. Upon graduation, he co‑founded a biotech company that re‑engineered virus capsids that served as a drug delivery platform. At Bio‑Rad, he manages the electrophoresis and western blotting product portfolio.

Reviews for this course

5 star rating

Excellent review on analysis and quantitation of your blot

Pauline Parkinson

This course demonstrates in detail the analysis and calculation of your target protein(s). I would recommend this course for all scientists involved in weste...

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This course demonstrates in detail the analysis and calculation of your target protein(s). I would recommend this course for all scientists involved in western blotting.

Read Less
5 star rating

The course is excellent and informative.

Reshma Reddy

It has helped me to clear doubts about normalization to lane. Thank you.

It has helped me to clear doubts about normalization to lane. Thank you.

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5 star rating

perfect webinar course

Khatab Mawlood

Thank you very much for this beneficial courses.

Thank you very much for this beneficial courses.

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5 star rating

Illuminating

Joshua Kleam

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FAQ

  • Who should take this course?

    This course is designed for people who want to have a fundamental understanding of gel electrophoresis and protein transfer.

  • How long will this course take?

    This class should take approximately 1 to 1.5 hours to complete.

  • Can I get a certificate for this course?

    Once all sections of this course have been completed a certificate with your name and course name will be available for download.

  • Will I be able to ask questions and get answers?

    By enrolling in this class you will be automatically added to Bio-Rad's Western Blotting Community. This is a highly collaborative place to ask questions and share western blotting ideas with the instructors and your peers. You can post pictures, comments and reply to others.

  • What are the other classes in this series?

    There are five classes in the Fundamentals of Western Blotting series. Course 1: Introduction to western blotting & sample prep, Course 2: Gel electrophoresis & transfer, Course 3: Immunodetection of target proteins, Course 4: Image acquisition, Course 5: Image analysis

All Courses

In the fundamentals of western blotting series

Course 1: Western Blotting Overview & Sample Preparation

Course 2: Gel Electrophoresis and Transfer

Course 3: Immunodetection

Course 4: Image Acquisition

Course 5: Image Analysis