Fundamentals of Western Blotting Image Acquisition
Course #4 of the Fundamentals of Western Blotting Series
This is course #4 of a 5-part fundamentals of western blotting series. These courses are for anyone who would like to have a detailed understanding of the principles of each step of the western blotting workflow.
Courses are led by Bio‑Rad’s Field Application Specialists who have diligently compiled all the questions they have received in the field and have designed this program to be both comprehensive and efficient.
You will receive a downloadable certificate upon completion of each course.
Course Overview
This is a preview, please enroll to access these lessons
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Welcome to the course
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How to use this course
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Meet the instructor
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Curriculum Overview
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Review of Immunoblotting
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Collection Considerations
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Quiz #1
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Overview of Acquisition Technologies (8 min)
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Quiz #2 Acquisition Technologies
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Film (4 min)
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Quiz #3 Film
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Laser Scanners (3 min)
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Quiz #4 Laser Scanners
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Densitometers (2 min)
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Quiz #5 Densitometer
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Colorimetric Basics (3 min)
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Quiz #6 Colorimetric Basics
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Chemiluminescence Basics (3 min)
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Quiz #7 Chemiluminescence Basics
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Fluorescence Image Acquisition (4 min)
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Quiz #8 Fluorescence Image Acquisition
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Multiplex Fluorescence (6 min)
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Quiz #9 Multiplex Fluorescence
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Image Acquisition Resources
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Get Your Free Western Blotting Workflow Poster
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About this course
- Free
- 25 lessons
- 1 hour of video content
Meet your instructor
Field Application Specialist Brad VanderWielen
Brad VanderWielen is currently a Field Application Specialist for Bio‑Rad Laboratories in the South‑Central region of the United States. He specializes in Chromatography, Western Blotting, and Bio‑Plex Multiplex ELISA instrumentation and associated experiments. He has been at Bio‑Rad for 5 years and has been in the field of Chromatography for the past 13 years. He came to Bio‑Rad from his postdoctoral work at UTSouthwestern Medical School where he was utilizing biophysics and X‑Ray crystallography to study tubulin regulation. Brad earned his PhD from the University of Cincinnati where he researched Notch transcriptional regulation utilizing biophysical applications and X‑Ray crystallography.
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Reviews for this course
Excellent Image Acquisition Fundamentals
Pauline Parkinson
This course emphasizes the different imaging techniques and how to optimize the detection of your signal with the blot. I recommend this course for any novic...
Read MoreThis course emphasizes the different imaging techniques and how to optimize the detection of your signal with the blot. I recommend this course for any novice or expert scientist.
Read LessVery well for troubleshootings
María Fernanda Vélez González
For me was very informative and easy to understand. Thanks
For me was very informative and easy to understand. Thanks
Read LessExcellent
Dr Vijayabaskar Pandian
its very nice and informative
its very nice and informative
Read LessExtremely technical
Joshua Kleam
FAQ
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Who should take this course?
This course is designed for people who want to have a fundamental understanding of gel electrophoresis and protein transfer.
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How long will this course take?
This class should take approximately 1.5 to 2 hours to complete.
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Can I get a certificate for this course?
Once all sections of this course have been completed a certificate with your name and course name will be available for download.
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Will I be able to ask questions and get answers?
By enrolling in this class you will be automatically added to Bio-Rad's Western Blotting Community. This is a highly collaborative place to ask questions and share western blotting ideas with the instructors and your peers. You can post pictures, comments and reply to others.
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What are the other classes in this series?
There are five classes in the Fundamentals of Western Blotting series. Course 1: Introduction to western blotting & sample prep, Course 2: Gel electrophoresis & transfer, Course 3: Immunodetection of target proteins, Course 4: Image acquisition, Course 5: Image analysis
All Courses
In the fundamentals of western blotting series