This is course #4 of a 5-part fundamentals of western blotting series. These courses are for anyone who would like to have a detailed understanding of the principles of each step of the western blotting workflow.  

Courses are led by Bio‑Rad’s Field Application Specialists who have diligently compiled all the questions they have received in the field and have designed this program to be both comprehensive and efficient.

You will receive a downloadable certificate upon completion of each course.

What you will learn

in Course #4: Fundamentals of Western Blotting Image Acquisition

  • Chemiluminescence and fluorescence detection in western blots

  • How to optimize your experiment to get the best possible sensitivity

  • Overview of imaging technologies

Learn the fundamentals of Western Blotting Image Acquisition

Course Overview

This is a preview, please enroll to access these lessons

    1. Welcome to the course

    2. How to use this course

    3. Meet the instructor

    4. Curriculum Overview

    1. Review of Immunoblotting

    1. Collection Considerations

    2. Quiz #1

    1. Overview of Acquisition Technologies (8 min)

    2. Quiz #2 Acquisition Technologies

    3. Film (4 min)

    4. Quiz #3 Film

    5. Laser Scanners (3 min)

    6. Quiz #4 Laser Scanners

    7. Densitometers (2 min)

    8. Quiz #5 Densitometer

    9. Colorimetric Basics (3 min)

    10. Quiz #6 Colorimetric Basics

    11. Chemiluminescence Basics (3 min)

    12. Quiz #7 Chemiluminescence Basics

    13. Fluorescence Image Acquisition (4 min)

    14. Quiz #8 Fluorescence Image Acquisition

    15. Multiplex Fluorescence (6 min)

    16. Quiz #9 Multiplex Fluorescence

    1. Image Acquisition Resources

    2. Get Your Free Western Blotting Workflow Poster

About this course

  • Free
  • 25 lessons
  • 1 hour of video content

Meet your instructor

Field Application Specialist Brad VanderWielen

Brad VanderWielen is currently a Field Application Specialist for Bio‑Rad Laboratories in the South‑Central region of the United States. He specializes in Chromatography, Western Blotting, and Bio‑Plex Multiplex ELISA instrumentation and associated experiments. He has been at Bio‑Rad for 5 years and has been in the field of Chromatography for the past 13 years. He came to Bio‑Rad from his postdoctoral work at UTSouthwestern Medical School where he was utilizing biophysics and X‑Ray crystallography to study tubulin regulation. Brad earned his PhD from the University of Cincinnati where he researched Notch transcriptional regulation utilizing biophysical applications and X‑Ray crystallography.

Reviews for this course

5 star rating

Excellent Image Acquisition Fundamentals

Pauline Parkinson

This course emphasizes the different imaging techniques and how to optimize the detection of your signal with the blot. I recommend this course for any novic...

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This course emphasizes the different imaging techniques and how to optimize the detection of your signal with the blot. I recommend this course for any novice or expert scientist.

Read Less
5 star rating

Very well for troubleshootings

María Fernanda Vélez González

For me was very informative and easy to understand. Thanks

For me was very informative and easy to understand. Thanks

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5 star rating


Dr Vijayabaskar Pandian

its very nice and informative

its very nice and informative

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5 star rating

Extremely technical

Joshua Kleam


  • Who should take this course?

    This course is designed for people who want to have a fundamental understanding of gel electrophoresis and protein transfer.

  • How long will this course take?

    This class should take approximately 1.5 to 2 hours to complete.

  • Can I get a certificate for this course?

    Once all sections of this course have been completed a certificate with your name and course name will be available for download.

  • Will I be able to ask questions and get answers?

    By enrolling in this class you will be automatically added to Bio-Rad's Western Blotting Community. This is a highly collaborative place to ask questions and share western blotting ideas with the instructors and your peers. You can post pictures, comments and reply to others.

  • What are the other classes in this series?

    There are five classes in the Fundamentals of Western Blotting series. Course 1: Introduction to western blotting & sample prep, Course 2: Gel electrophoresis & transfer, Course 3: Immunodetection of target proteins, Course 4: Image acquisition, Course 5: Image analysis