Fundamentals of Western Blotting Immunodetection
Course #3 of the Fundamentals of Western Blotting Series
This is course #3 of a 5-part fundamentals of western blotting series. These courses are for anyone who would like to have a detailed understanding of the principles of each step of the western blotting workflow.
Courses are led by Bio‑Rad’s Field Application Specialists who have diligently compiled all the questions they have received in the field and have designed this program to be both comprehensive and efficient.
You will receive a downloadable certificate upon completion of each course.
This is a preview, please enroll to access these lessons
Welcome to the course
How to use this course
Meet the instructor
Immunodetection workflow
Quiz 1
Primary Antibody Validation
Quiz #2
Primary Antibody Optimization
Quiz #3
Secondary Antibody
Quiz #4
Linear Dynamic Range of a Western Blot
Quiz #5
The course was detailed in the principles of immunodetection. An excellent review of the steps is given and troubleshooting is also reviewed. An excellent co...
Read MoreThe course was detailed in the principles of immunodetection. An excellent review of the steps is given and troubleshooting is also reviewed. An excellent course for the novice as well as the expert scientist. I recommend this course for its details and troubleshooting on immunodetection.
Read LessNice easy to understand
Nice easy to understand
Read LessHighly advandaced and validated course makes a researcher able to choose and do the best thing
Highly advandaced and validated course makes a researcher able to choose and do the best thing
Read LessIt has a good content
It has a good content
Read LessThis course is designed for people who want to have a fundamental understanding of gel electrophoresis and protein transfer.
This class should take approximately 1 to 1.5 hours to complete.
Once all sections of this course have been completed a certificate with your name and course name will be available for download.
By enrolling in this class you will be automatically added to Bio-Rad's Western Blotting Community. This is a highly collaborative place to ask questions and share western blotting ideas with the instructors and your peers. You can post pictures, comments and reply to others.
There are five classes in the Fundamentals of Western Blotting series. Course 1: Introduction to western blotting & sample prep, Course 2: Gel electrophoresis & transfer, Course 3: Immunodetection of target proteins, Course 4: Image acquisition, Course 5: Image analysis
In the fundamentals of western blotting series