Running Your First ddPCR Experiment

Key Considerations for Success

Excited to start your first ddPCR experiment? Before you begin, it’s essential to review best practices to ensure accurate and reliable results. This course will guide you through crucial aspects of sample and reaction setup, proper data analysis for single and duplex reactions, and effective data interpretation and management. While some of these principles may seem straightforward, others are often overlooked until they become an issue. By the end of this module, you’ll have the foundational knowledge to confidently run and analyze your ddPCR experiments.

What you will learn

in Course #3: Running Your First Droplet Digital PCR Experiments

  • General sample and reaction setup considerations.

  • Proper data analysis for single and duplex reactions.

  • Data interpretation and management.

Course Curriculum

    1. Welcome to Introduction to Running Your First Set of Droplet Digital PCR (ddPCR) Experiments

    2. How to Use this Course

    3. Meet the Instructor

    4. Introduction to Running Your First Set of ddPCR Experiments

    1. Reaction Prep

    2. Chapter 1 Quiz

    1. Plate Setup

    2. Chapter 2 Quiz

    1. Data Analysis

    2. Chapter 3 Quiz

    1. Data & Recap

    2. Chapter 4 Quiz

    3. Recap & Resources

About this course

  • Free
  • 5 Chapters, approx. 45 min

Discover your potential, starting today

Meet Your Instructor

Frank Bizouarn

Your instructor for this course is Frank Bizouarn. Frank joined Bio-Rad in 2000 to support quantitative PCR (qPCR) technology as a field applications specialist (FAS) in the southeast United States. In 2006, he moved into the role of International FAS for Bio-Rad’s global Gene Expression Division to promote best practices in qPCR around the world. In 2011, Frank began working on droplet digital PCR applications (ddPCR) and joined the Digital Biology group. Currently, he is focused on supporting and promoting quantitation, detection, and sample discrimination applications that take advantage of the high resolution and sensitivity provided by the power of droplet partitioning.

FAQ

  • Who should take this course?

    This course is designed for people who want to have a fundamental understanding of digital PCR.

  • How long will this course take?

    This class should take approximately 45 minutes to complete.

  • What are the other courses in this series?

    There are six classes in the Fundamentals of Digital PCR series. Our scientists are working hard to build more courses, please stayed tune for updates.

More Digital PCR Courses Are Coming

Sign up and stay up to date on the latest digital PCR courses >