As we continue to fine refine cause/effect analyses using Gene Expression, ddPCR offers a set of unique analysis parameters that allow researchers to push these boundaries further and further. Accurate absolute quantification (without standards), tolerance to inhibition, simplified analysis, and higher order multiplexing… to name a few will help you generate Gene Expression analysis results that are much more impactful. 

This course explains how digital PCR works,  covers ddPCR assay design, migration of assays onto ddPCR, discusses reverse transcription, multiplexing and normalization considerations, and describes best practices in data analysis.

Your team members at Bio-Rad’s Digital Biology Group along with a global team of Field Application Scientists have been developing new instrumentation and reagents, designing novel applications and supporting ddPCR users to push the boundaries of this technology for over a decade. We have learned much throughout this journey and want to share this knowledge with you to help extend these boundaries even further.  

What will your data tell you today? (we can help).

What you will learn

Course Extension: Gene Expression Using Digital PCR

  • How digital PCR works

  • ddPCR assay design

  • Multiplexing and normalization considerations

  • Best practices in data analysis

Course Overview

This is a preview, please enroll to access these lessons

    1. Welcome to Course Extension: Gene Expression Using Digital PCR

    2. How to use this course

    3. Meet the instructor

    4. Curriculum Overview

    1. What is Digital PCR

    2. Quiz #1

    1. Counting with Digital PCR

    2. Quiz #2

    1. Assay Design Considerations

    2. Quiz #3

    1. Assay Migration and Optimization

    2. Quiz #4

    1. Multiplexing

    2. Quiz #5

About this course

  • Free
  • 22 lessons
  • 1 hour of video content

Meet your instructor

Frank Bizouarn

Your instructor for this course is Frank Bizouarn. Frank joined Bio-Rad in 2000 to support quantitative PCR (qPCR) technology as a field applications specialist (FAS) in the southeast United States. In 2006, he moved into the role of International FAS for Bio-Rad’s global Gene Expression Division to promote best practices in qPCR around the world. In 2011, Frank began working on droplet digital PCR applications (ddPCR) and joined the Digital Biology group. Currently, he is focused on supporting and promoting quantitation, detection, and sample discrimination applications that take advantage of the high resolution and sensitivity provided by the power of droplet partitioning.


  • Who should take this course?

    This course is designed for people who want to have a fundamental understanding of digital PCR.

  • How long with this course take?

    This class should take approximately 1 hour to complete.

  • Can I get a certificate for this course?

    Once all sections of this course have been completed a certificate with your name and course name will be available for download.

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