As we continue to fine refine cause/effect analyses using Gene Expression, ddPCR offers a set of unique analysis parameters that allow researchers to push these boundaries further and further. Accurate absolute quantification (without standards), tolerance to inhibition, simplified analysis, and higher order multiplexing… to name a few will help you generate Gene Expression analysis results that are much more impactful. 

This chapter focuses on Gene Expression and covers ddPCR assay design, migration of assays onto ddPCR, reverse transcription, multiplexing and normalization considerations, and describes best practices in data analysis in relation to gene expression.

What will your data tell you today? (we can help).

What you will learn

in Course #2: Gene Expression Using Droplet Digital PCR

  • ddPCR assay design

  • Multiplexing and normalization considerations

  • Best practices in data analysis

Course Overview

This is a preview, please enroll to access these lessons

    1. Welcome to Introduction to Gene Expression Using Digital PCR

    2. How to use this course

    3. Meet the instructor

    4. Curriculum Overview

    1. Assay Design Considerations

    2. Quiz #1

    1. Assay Optimization

    2. Quiz #2

    1. Multiplexing

    2. Quiz #3

    1. Prep & RT Considerations

    2. Quiz #4

    1. Normalization

    2. Quiz #5

About this course

  • Free
  • 18 lessons
  • 1 hour of video content

Meet your instructor

Frank Bizouarn

Your instructor for this course is Frank Bizouarn. Frank joined Bio-Rad in 2000 to support quantitative PCR (qPCR) technology as a field applications specialist (FAS) in the southeast United States. In 2006, he moved into the role of International FAS for Bio-Rad’s global Gene Expression Division to promote best practices in qPCR around the world. In 2011, Frank began working on droplet digital PCR applications (ddPCR) and joined the Digital Biology group. Currently, he is focused on supporting and promoting quantitation, detection, and sample discrimination applications that take advantage of the high resolution and sensitivity provided by the power of droplet partitioning.


  • Who should take this course?

    This course is designed for people who want to have a fundamental understanding of digital PCR.

  • How long with this course take?

    This class should take approximately 1 hour to complete.

  • Can I get a certificate for this course?

    Once all sections of this course have been completed a certificate with your name and course name will be available for download.

  • What are the other classes in this series?

    There are six classes in the Fundamentals of Digital PCR series. Our scientists are working hard to build more courses, please stayed tune for updates.