This is course 4 of a seven-part Antibody Essentials series. It is aimed at anyone who works with antibodies and would like to build their knowledge of antibody best practices to optimize their workflows and achieve reliable results. Courses are led by Bio-Rad’s antibody experts who share their knowledge and advice to deliver a program that is effective and comprehensive in a way that is clear to antibody users of all experience levels. Upon completion of each course, you will receive a downloadable certificate and gain access to Bio-Rad’s Antibody Community, in which you can collaborate with and get guidance from both your peers and our antibody experts.

What you will learn

Course 4: Selecting Your Secondary Antibody

  • The difference between direct and indirect detection methods

  • What features you need to consider when choosing a secondary antibody

  • The purpose of cross-adsorption

Course Curriculum

    1. Welcome to the Course

    2. How to Use this Course

    3. Meet the Instructor

    1. Selecting Your Secondary Antibody

    2. Course 4 Questions

    1. Useful Guides and Tools

About this course

  • Free
  • 1 Course, 20 mins

Meet Your Instructor

Technical Editor Flow Cytometry and Antibodies Chloe Fenton

Your instructor for this course is Chloe Fenton, PhD. Chloe graduated from the University of Manchester, UK, with a degree in Biomedical Sciences. She then carried out her Master’s in immunology at Newcastle University, UK, before moving to the University of Birmingham, UK, to complete her PhD thesis investigating the role of pre-receptor steroid metabolism in the anti-inflammatory effects of therapeutic glucocorticoids. In 2020, she moved to the University of Wuerzburg, Germany, for an immunology postdoctoral position focusing on how regulatory T cells suppress CD8 T cell function during viral infections. Chloe joined Bio-Rad in 2023 as a Technical Editor in Flow Cytometry and Antibodies.

FAQ

  • Who should take this course?

    This course is designed for people who want to have a fundamental understanding of how to store, handle, and use antibodies to obtain optimal results in their experiments.

  • How long will this course take?

    This class should take approximately 20 minutes to complete.

  • Can I get a certificate for this course?

    Once all sections of this course have been completed a certificate with your name and course name will be available for download.

  • Will I be able to ask questions and get answers?

    By enrolling in this class you will be automatically added to Bio-Rad's Antibody Community. This is a highly collaborative place to ask questions and share ideas with the instructors and your peers. You can post pictures, comments, and reply to others.

  • What are the other classes in this series?

    There are seven classes in the Antibody Essentials Series: Course 1: Handling Your Antibody; Course 2: Working with Your Antibody — Protocols; Course 3: Selecting Your Primary Antibody; Course 4: Selecting Your Secondary Antibody; Course 5: Working with Your Antibody — Titration; Course 6: Working with Your Antibody — Controls Part 1; Course 7: Working with Your Antibody — Controls Part 2